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epi fluorescence illuminator  (Nikon)


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    Structured Review

    Nikon epi fluorescence illuminator
    Epi Fluorescence Illuminator, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 723 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/epi+fluorescence+illuminator/Epi-Fl+LED+Illuminator/arxiv__2512__23123-48-15-17
    Average 96 stars, based on 723 article reviews
    epi fluorescence illuminator - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Metrology and characterization of SU-8 microstructures using autofluorescence emission
    Article Snippet: .. Fluorescence imaging The autofluorescence emission of the SU-8 microstructures fabricated on both borosilicate glass wafers and silicon wafers was measured using an epi-fluorescence illuminator (Nikon Intensilight C-HGFI, Tokyo, Japan), an inverted microscope (Nikon Eclipse Ti-E, Tokyo, Japan), and a DAPI filter set (peak excitation wavelength of 365 nm; peak emission wavelength of 440 nm). .. Images were captured with a 10× objective and a microscope camera (Andor Zyla 5.5sCMOS, Abingdon, UK) with an exposure time of 30 s in a dark room to prevent interference from radiation in the visible spectrum.

    Article Title: [2512.23123] Understanding the mechanisms of supported lipid membrane reshaping into tubular networks using quantitative DIC microscopy
    Article Snippet: .. For fluorescence measurements, a metal-halide high pressure lamp (Prior Lumen 200) was used with a epi-fluorescence illuminator (Nikon Ti-FL), filter turret (Nikon Ti-FLC), and a filter cube (Semrock GFP-ABASIC, excitation 35 nm wide centred at 469 nm, emission 39 nm wide centred at 525 nm). .. For differential interference contrast (DIC), a 100 W halogen lamp (Nikon Ti-DH D-LH/LC) was used with a green interference filter (Nikon GIF) to provide a illumination wavelength centred at 550 nm and a full-width at half maximum (FWHM) of 53 nm.

    Article Title: Combined High‐Speed Atomic Force and Optical Microscopy Shows That Viscoelastic Properties of Melanoma Cancer Cells Change during the Cell Cycle
    Article Snippet: .. Optical Setup: The AFM was mounted on a commercial optical microscope (Eclipse TI-S, Nikon Corporation, Tokyo, Japan) with a changeable objective (20×, NA 0.45, Ph1, Nikon), a fluorescence module using an epi-fluorescence illuminator (C-HGFI Intensilight, Nikon), and a low-noise CMOS camera (DS-Qi2, Nikon). .. Incubator System: For sensitive cells (Figures 4 and 5), the sample holder could be optionally equipped with a silicone lid to close the cell culture dish and connected to a commercial gas incubation system (11922, ibidi GmbH, Martinsried, Germany) and air pressure generator (11929, ibidi GmbH) to keep the cells in their normal cell culture medium under cell culture conditions at 36 or 37 °C with 5% CO2 (v/v).

    Imaging:

    Article Title: Metrology and characterization of SU-8 microstructures using autofluorescence emission
    Article Snippet: .. Fluorescence imaging The autofluorescence emission of the SU-8 microstructures fabricated on both borosilicate glass wafers and silicon wafers was measured using an epi-fluorescence illuminator (Nikon Intensilight C-HGFI, Tokyo, Japan), an inverted microscope (Nikon Eclipse Ti-E, Tokyo, Japan), and a DAPI filter set (peak excitation wavelength of 365 nm; peak emission wavelength of 440 nm). .. Images were captured with a 10× objective and a microscope camera (Andor Zyla 5.5sCMOS, Abingdon, UK) with an exposure time of 30 s in a dark room to prevent interference from radiation in the visible spectrum.

    Inverted Microscopy:

    Article Title: Metrology and characterization of SU-8 microstructures using autofluorescence emission
    Article Snippet: .. Fluorescence imaging The autofluorescence emission of the SU-8 microstructures fabricated on both borosilicate glass wafers and silicon wafers was measured using an epi-fluorescence illuminator (Nikon Intensilight C-HGFI, Tokyo, Japan), an inverted microscope (Nikon Eclipse Ti-E, Tokyo, Japan), and a DAPI filter set (peak excitation wavelength of 365 nm; peak emission wavelength of 440 nm). .. Images were captured with a 10× objective and a microscope camera (Andor Zyla 5.5sCMOS, Abingdon, UK) with an exposure time of 30 s in a dark room to prevent interference from radiation in the visible spectrum.

    Article Title: Metrology and characterization of SU-8 microstructures using autofluorescence emission
    Article Snippet: .. The autofluorescence emission of the SU-8 microstructures fabricated on both borosilicate glass wafers and silicon wafers was measured using an epi-fluorescence illuminator (Nikon Intensilight C-HGFI, Tokyo, Japan), an inverted microscope (Nikon Eclipse Ti-E, Tokyo, Japan), and a DAPI filter set (peak excitation wavelength of 365 nm; peak emission wavelength of 440 nm). ..

    Microscopy:

    Article Title: Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract
    Article Snippet: Glass rod Fisher 50-121-5269 MitoTracker CMXRos Fisher M7512 Shield from light, store at −20°C Monopostassium phosphate Fisher P285-500 Disodium phosphate Fisher S374-1 Magnesium sulfate Fisher M63–500 Dimethyl sulfoxide Fisher BP231–1 DMSO Aluminum foil Fisher 01-213-102 Ethyl 3-aminobenzoate methanesulfonate Sigma E10521-10G Tricaine is the common name Tetramisole hydrochloride Sigma L9756-5G Agarose Fisher BP1356–100 Coverslips Fisher 12-548-A 18 × 18-1 Frosted microscope slides Fisher 12-552-3 Equipment 16°C and 20°C incubators Fisher 97–990E Same model, set at different temperatures. .. Upright Microscope with epi-fluorescence illuminator, camera, and 10x and 60x objectives Refer to the protocol section for specific model information Software with image acquisition and tracking capabilities Protocol given based on the NIS-Elements software Stereo-microscope Nikon SMZ800N Any stereo-microscope that can be used to visualize C. elegans may be used with this protocol Open in a separate window NOTE: Mito-dye is light sensitive. ..

    Article Title: WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin
    Article Snippet: .. The microscope setup for photoactivation experiments was the same as that for TIRF microscopy except that the epi-fluorescence illuminator was used (Intensilight; Nikon) and the 1.5× intermediate lens was omitted. .. Photoactivation of PA-GFP-tubulin at designated regions in the spindle was achieved using Nikon Galvo Miniscanner with a 405-nm laser.

    Article Title: Combined High‐Speed Atomic Force and Optical Microscopy Shows That Viscoelastic Properties of Melanoma Cancer Cells Change during the Cell Cycle
    Article Snippet: .. Optical Setup: The AFM was mounted on a commercial optical microscope (Eclipse TI-S, Nikon Corporation, Tokyo, Japan) with a changeable objective (20×, NA 0.45, Ph1, Nikon), a fluorescence module using an epi-fluorescence illuminator (C-HGFI Intensilight, Nikon), and a low-noise CMOS camera (DS-Qi2, Nikon). .. Incubator System: For sensitive cells (Figures 4 and 5), the sample holder could be optionally equipped with a silicone lid to close the cell culture dish and connected to a commercial gas incubation system (11922, ibidi GmbH, Martinsried, Germany) and air pressure generator (11929, ibidi GmbH) to keep the cells in their normal cell culture medium under cell culture conditions at 36 or 37 °C with 5% CO2 (v/v).

    Software:

    Article Title: Measuring Sperm Guidance and Motility within the Caenorhabditis elegans Hermaphrodite Reproductive Tract
    Article Snippet: Glass rod Fisher 50-121-5269 MitoTracker CMXRos Fisher M7512 Shield from light, store at −20°C Monopostassium phosphate Fisher P285-500 Disodium phosphate Fisher S374-1 Magnesium sulfate Fisher M63–500 Dimethyl sulfoxide Fisher BP231–1 DMSO Aluminum foil Fisher 01-213-102 Ethyl 3-aminobenzoate methanesulfonate Sigma E10521-10G Tricaine is the common name Tetramisole hydrochloride Sigma L9756-5G Agarose Fisher BP1356–100 Coverslips Fisher 12-548-A 18 × 18-1 Frosted microscope slides Fisher 12-552-3 Equipment 16°C and 20°C incubators Fisher 97–990E Same model, set at different temperatures. .. Upright Microscope with epi-fluorescence illuminator, camera, and 10x and 60x objectives Refer to the protocol section for specific model information Software with image acquisition and tracking capabilities Protocol given based on the NIS-Elements software Stereo-microscope Nikon SMZ800N Any stereo-microscope that can be used to visualize C. elegans may be used with this protocol Open in a separate window NOTE: Mito-dye is light sensitive. ..



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    Nikon nikon epi fluorescence microscope
    Scheme of the combined IPM-confocal <t>fluorescence</t> system used for imaging. The IPM beams appear in red; The fluorescent excitation beam appears in blue; The fluorescent emission beam appears in purple; The BFM beam appears in gray. CO, condenser lens; MO, microscope objective; BS, beam splitter; L1, L2, L3, L4, L5, L6, L7, L8 lenses; SD spinning disk; DM dichroic mirror; C1, C2, digital cameras; DG, diffraction grating; Ma, mask; C, compensation glass; M, mirror; BE, blue emission; BF, brightfield.
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    Image Search Results


    Scheme of the combined IPM-confocal fluorescence system used for imaging. The IPM beams appear in red; The fluorescent excitation beam appears in blue; The fluorescent emission beam appears in purple; The BFM beam appears in gray. CO, condenser lens; MO, microscope objective; BS, beam splitter; L1, L2, L3, L4, L5, L6, L7, L8 lenses; SD spinning disk; DM dichroic mirror; C1, C2, digital cameras; DG, diffraction grating; Ma, mask; C, compensation glass; M, mirror; BE, blue emission; BF, brightfield.

    Journal: Biomedical Optics Express

    Article Title: Label-free refractive index mapping of human sperm cells

    doi: 10.1364/BOE.567655

    Figure Lengend Snippet: Scheme of the combined IPM-confocal fluorescence system used for imaging. The IPM beams appear in red; The fluorescent excitation beam appears in blue; The fluorescent emission beam appears in purple; The BFM beam appears in gray. CO, condenser lens; MO, microscope objective; BS, beam splitter; L1, L2, L3, L4, L5, L6, L7, L8 lenses; SD spinning disk; DM dichroic mirror; C1, C2, digital cameras; DG, diffraction grating; Ma, mask; C, compensation glass; M, mirror; BE, blue emission; BF, brightfield.

    Article Snippet: It consists of a commercial inverted microscope (Olympus PlanApo N 60×/1.42 oil), along with an epi-illuminance fluorescence spinning-disk confocal imaging unit (CSU-X1, Yokogawa, Tokyo, Japan) and a custom-built external interferometric module integrated at the microscope's output.

    Techniques: Fluorescence, Imaging, Microscopy